Subsequently, a least absolute shrinkage and selection operator (LASSO) Cox regression analysis was implemented based on the 53 genes to establish the prognostic signature for glioma patients, identifying 11 key genes (Figure 3a): PLOD1, CCR5, CTSZ, ITGB2, TLR2, ASPM, GINS4, KIF14, KIF2C, KPNA2, and POLD3. Here, POLD3 is linked to central nervous system cancer.