Two key proteins, Bax and Bcl-2, involved in the regulation of apoptosis, were analyzed using the RT-qPCR method; the results showed that recombinant virus infection upregulated Bax and downregulated Bcl-2 in B16F10 cells, indicating an increased propensity for apoptosis (Figure 3a,b). Here, BCL2 is linked to viral infectious disease.