CYP19A1 and colorectal carcinoma: Furthermore, colony formation and 5-ethynyl-2’-deoxyuridine (EdU) incorporation assays confirmed that CYP19A1 significantly enhances the proliferative capacity of CRC cells, as demonstrated by a marked reduction in colony numbers (Figure 5F) and a decrease in EdU incorporation into replicating DNA (Figure 5G,H).