Both miRNAs showed valuable diagnostic parameters in discriminating HCC patients from healthy controls and patients with other liver diseases (AUC 0.759 p < 0.001 and AUC 0.798 p < 0.001 for miR-122 and miR-16, respectively), further improved by combining the two miRNAs with AFP (AUC 0.862, p < 0.001). This evidence concerns the gene AFP and hepatocellular carcinoma.