A study that examined the single-cell RNA-seq method to characterize dynamic changes in TME of PDAC patients charted the emergence of four subsets of macrophages during malignancy, in which MARCO was co-expressed with SPP1 mRNA marker gene [19] pro-angiogenic TAMs in the development of PDAC [26], playing a role in epithelial–mesenchymal transition (EMT) and aiding in tumor invasion [21]. This evidence concerns the gene SPP1 and neoplasm.