Future research to strengthen the findings of our study and experiments to validate the demonstrated differential expression observed in obesity and its relevance to specific cell types could include techniques such as in situ hybridization, confocal microscopy, and validation of specific genes identified in the present study (such as Nr1d1, Slc39a13, Ddc, Tppp, Stat5b, Fam214a, Tnrc6b, and Muc6). This evidence concerns the gene NR1D1 and obesity due to melanocortin 4 receptor deficiency.