Obesity mostly upregulated DEGs in endothelial cells for pathways regulating endothelial permeability such as focal adhesion (Prkcb and Vegfc), adherens junction (Pard3), leukocyte transendothelial migration (Prkcb and Vcam1), and Rap1 signaling (Pard3, Prkcb, and Vegfc). This evidence concerns the gene VCAM1 and obesity due to melanocortin 4 receptor deficiency.