ACTA1 and metabolic syndrome: To elucidate whether L-arginine (NO precursor) treatment improved bladder angiogenesis in a rat model of MetS with or without OHD-induced detrusor hyperactivity, angiogenesis-related markers (alpha smooth muscle actin (α-SMA), Laminin, and VEGF) were quantified by immunostaining (Figure 6A–G) and Western Blots (Figure 6H,I).