In contrast, α-SMA immunostaining was prominently expressed in the myofibroblasts, microvessels, and vessels of the urothelial basal layer, SL, and ML in the MetS + L-arginine group (Figure 6C), the MetS + L-NAME group (Figure 6D), the MetS + OVX + L-arginine group (Figure 6F), and the MetS + OVX + L-NAME group (Figure 6G), compared to the MetS and MetS + OVX groups. The gene discussed is ACTA1; the disease is metabolic syndrome.