Consistent with the gene expression data, immunofluorescence staining of 3-day-old tumor spheroids from the RPM culture confirmed that Calu-3 spheroids are mainly characterized by less β1-integrin and E-cadherin and much more mucin-1 and laminin compared to NCI-H1703 spheroids at this stage (Figure 3C). The gene discussed is MUC1; the disease is neoplasm.