Despite similar levels of HBx expression in both WT HBV and HBV S101P-infected cells after 1 day of infection, the effects of WT HBV on p53, Mn-SOD, catalase, and ROS levels were significantly higher (Figure 6E,G), highlighting the crucial role of the Ser-101 residue in HBx for p53 activation and the consequent upregulation of ROS levels. This evidence concerns the gene CAT and infection.