NUCB2 and pyometritis: Plasma NUCB2/nesfatin-1 concentrations of the healthy female dogs and those suffering from CEH and pyometra are shown in Fig. 3A. Nesfatin-1 concentration was increased in the blood of diestrus dogs (C2 group) or affected by uterus disorders (CEH2 and P2 groups) and with confirmed overweight, compared to animals with BCS = 4/9 (C1, CEH1, P1 groups) (p < 0.05).