In contrast to the Neo1-ECD/TMD as a predominant form of Neo1 in the liver of WT mice (Fig. 5H) (28), the relatively low levels of Neo1-ECD/TMD detected in hepatoma cells are likely attributed to the limitation of overexpression model used in the study, as well as the lack of Hjv and other hepcidin-inducing proteins. This evidence concerns the gene NEO1 and hepatocellular carcinoma.