Thus considering this all, in the present study we have employed various bioinformatics approaches such as functional analysis (antigenicity, allergenicity, antigen binding sites & signalling cascades), qualitative analysis (physicochemical, prediction, refinement & validation of 2D and 3D structures), molecular docking, and In silico cloning to design and develop the novel CAR protein to be used in engineering the T-cell to elucidate the antitumor response against KRAS-mutated pancreatic ductal adenocarcinoma. This evidence concerns the gene KRAS and pancreatic ductal adenocarcinoma.