We have thus provided experimental evidence involving MHC-associated peptide proteomic assays of antigen-presenting cells regarding (i) the number of unique infection-derived peptides isolated and such peptides’ average length; (ii) the derived peptides for each genotyped subject and motifs identified, showing that affinities for MHC-II specific variants varied between individuals; (iii) S. thypimurium pathogen-specific peptides; and (iv) binding specificities or in silico binding predictions for evaluating overall dataset quality. The gene discussed is HLA-C; the disease is infection.