Finally, in the McGill-R-Thy1-APP heterozygous rat model of AD, expressing human APP with three familial mutations (K607N, M671L, and I716V), beginning at 1 month of age and following 6.5 months of intervention demonstrated increased systemic insulin levels and increased accumulation of amyloid-beta in hippocampal tissue [409]. This evidence concerns the gene APP and Alzheimer disease.