Our results showed a significant downregulation of TLR4 in HPV-related H-SIL and SCC samples, compared to NILM, demonstrated by the substantial decrease in TLR4-SI in H-SIL and SCC samples compared to the non-neoplastic epithelium (p < 0.05, ANOVA). Here, TLR4 is linked to squamous cell intraepithelial neoplasia.