Another approach is obtained by combining cultures of different cells in the same model: HCC organoids cocultured with endothelial cells and fibroblasts expressed markers of vascularization, such as vimentin, vascular endothelial growth factor receptors (VEGFR) 2, hypoxia-inducible factor alpha 1 (HIF-1 alpha), and vascular endothelial growth factor (VEGF). This evidence concerns the gene HIF1A and hepatocellular carcinoma.