For the in vitro functional studies, initially, we selected two specific CRC cell lines (HT29 and SW480) exhibiting resistance to 5-Fu with high PLK1 expression, along with two other cell lines (DLD1 and HCT116) that were sensitive to 5-Fu with low expressions for PLK1 (Figure 3A,B). This evidence concerns the gene PLK1 and colorectal carcinoma.