Lentiviral vectors containing the Vβ 6.7 promoter were found to be optimal for TCR gene expression, maintaining expression for up to 7 weeks. These transduced T cells effectively recognized EBV antigens, demonstrated by their cytotoxicity and IFN-γ secretion. Additionally, mice infused with these cells showed significant resistance to LMP2-positive NPC cells. Here, IFNG is linked to nasopharyngeal carcinoma.