We thus investigated the transduction efficacy of LV.POR3‐RFP (Figure 1A) in the murine hepatoma cell line Hepa1‐6 by determining the insertion frequency and the cutting efficiency, that is, inactivation of Cypor by the SpCas9 and the corresponding anti‐Cypor gRNA (see Section 3.1 for selection of the gRNA). The gene discussed is POR; the disease is hepatocellular carcinoma.