To validate the above findings in vivo, PB‐derived neutrophils were treated with TTCS with or without G‐CSF and/or GM‐CSF‐neutralizing antibody or anti‐Mcl‐1 antibody and then co‐cultured with autologous tumor‐specific CD8+ T lymphocytes for 72 h. This evidence concerns the gene CSF3 and neoplasm.