CD274 and neoplasm: Flow cytometry was used to determine neutrophil function by focusing on the expression of markers associated with immunosuppression, such as PD‐L1,[18] PD‐L2,[32] and Siglec F.[23] Based on flow cytometry, the t‐SNE algorithm was applied to autologous peripheral blood (PB) and non‐tumor and tumor tissues from 15 patients with LSCC.