Furthermore, mice in SC had overall greater brain-wide activity in response to an acute injection of nicotine than mice in HC (Nicotine HC versus Nicotine SC, Fig 6C), including significant increases in cFos positive cells in regions associated with addiction, such as the medial prefrontal cortex, as well as in parts of the amygdala associated with anxiety and anxiety-like behavior. The gene discussed is FOS; the disease is Anxiety.