ICOShiBhlhe40hiCD4Th1, CD4Th1_A, and CD4cycling displayed features of neoAg-specific conventional CD4 T cells (isolated from human melanoma) that were previously described and phenotypically annotated (Oliveira_2022_Clust6_CD4.neoAg.Term.Exhaust, Oliveira_2022_Clust3_CD4.Follic/Progen.Exhaust, and Oliveira_2022_Clust8_CD4.neoAg.Proliferating)67 (Figure 5C). This evidence concerns the gene CD4 and melanoma.