The Cignal Reporter Assay Kit was employed to explore the potential molecular mechanism(s) of the NEDD4/FLRT2 axis in NSCLC cell stemness.The stemness-related signaling pathways, such as HIF-1α (Gu et al., 2021), NF-kB (Xu et al., 2024), and TCF/LEF (Katoh and Katoh, 2022) were identified as potential downstream pathways of this axis. The gene discussed is FLRT2; the disease is non-small cell lung carcinoma.