In present study, AA mice model with GSK-3β gene knockdown was used to further explain the mechanism of MOIG for treating RA based GSK-3β, the results exhibited inhibition of GSK-3β mitigated the RA symptom and activation of NF-κB and STAT3 signaling pathway, and MOIG treatment had no effects on AA mice with GSK-3β gene knockdown as evidenced arthritis clinical scores, spleen index, joint swelling, H&E staining, as well as cytokines factors and expression of p-p65 and p-STAT3 in AA mice, consisting with the in vitro experimental results. Here, GSK3B is linked to arthritic joint disease.