After Bclaf1 overexpression was confirmed by Western blotting, the expression of Bcl-2, a key protein of mitochondrial apoptosis in hepatoma cells, was increased and that of Bax was decreased, and the Bcl-2/Bax ratio was increased compared with the findings in the blank control group (all p < 0.01). The gene discussed is BAX; the disease is hepatocellular carcinoma.