To further confirm whether Tim-1 promotes HCC development by mediating cancer cell proliferation and regulating the ECM, we conducted an experiment in which 107 Hepa1-6 cells, 107 Hepa1-6sh-Tim-1 cells, 107 Hepa1-6+105 JS1 cells, and 107 Hepa1-6+105 JS1OE-Tim-1 cells were injected into the subcutaneous tissue of nude mice to establish a subcutaneous tumor model. The gene discussed is HAVCR1; the disease is cancer.