APC and colorectal carcinoma: To investigate whether the nature of CTNNB1 or APC mutations impact the stability and ubiquitylation of β-Catenin in CRC, did we conduct an endogenous ubiquitin TUBE (tandem ubiquitin binding entity) assay in a panel of human CRC lines, comprising β-Catenin mutant lines (HCT116 and LS174T), or cell lines varying in the truncation length of APC, DLD-1, SW480, SW620, Colo320 and HT-29, respectively (Fig. 1a).