To discover such regulators, we performed a human DUB siRNA screen in the APC truncated CRC line HT-29, followed by assessing the residual protein abundance of endogenous β-Catenin by immunofluorescence imaging using a high content screening microscope setup, relative to siRNA control transfected HT-29 (Fig. 1b, c). Here, ZUP1 is linked to colorectal carcinoma.