USP10 and colorectal carcinoma: To this end, we co-immunoprecipitated endogenous USP10 and β-Catenin in the human CRC lines HCT116 (CTNNB1mutant/APCwildtype) and HT-29 (CTNNB1wildtype/APCtruncated) by either immunoprecipitating USP10 or β-Catenin first, followed by probing against the putative novel interaction partner (Fig. 3a, b).