Given that viral infection and Spike protein could induce the expression of type I and II interferons (IFNs), we treated Caco-2 monolayer cultures with recombinant human IFN-α (IFN-α 2a), IFN-β, IFN-γ, or IFN-λ proteins for 24 h and quantified LSR mRNA levels using RT-qPCR. The gene discussed is IFNB1; the disease is viral infectious disease.