The results from the Annexin V-FITC Apoptosis Detection assay indicated that the silencing of circSP3 enhances cellular apoptosis, as depicted in Figure 3G. Wound healing assays and transwell assays revealed a significant decrease in migration and invasion of ccRCC cells following knockdown of circSP3 (Figure 4A-D). The gene discussed is ANXA5; the disease is nonpapillary renal cell carcinoma.