CHK1 was related to lymph node metastasis in colorectal cancer, gastric cancer, gallbladder cancer, esophageal squamous cell carcinoma, cervical carcinoma, and tongue squamous cell carcinoma, but not in bladder urothelial carcinoma, clear cell renal cell carcinomas, or breast cancer. The gene discussed is CHEK1; the disease is esophageal squamous cell carcinoma.