Western blot analysis revealed that the overexpression of miR-221-5p resulted in a significant decrease in the GSDMD protein level (Figure 3G), and a cellular rescue assay confirmed that the overexpression of GSDMD weakened the proliferative effect of miR-221-5p on CRC cells (Figure 3H,I). The gene discussed is GSDMD; the disease is colorectal carcinoma.