Furthermore, we conducted a Pearson test on the correlations of the genes and found that LINC00365 is positively correlated with miR-221-5p expression in colorectal cancer tissue and that miR-221-5p is negatively correlated with GSDMD expression; on this basis, we speculate that LINC00365 possibly inhibits pyroptosis by regulating the expression of GSDMD through the modulation of miR-221-5p. The gene discussed is GSDMD; the disease is colorectal cancer.