Furthermore, Nr5a2 expression exhibited a significant decrease in the livers of BKS-db mice, which serve as a model of type 2 diabetes and NASH (Fig. 1D–F), as well as in the livers of mice induced by high-fat diet (HFD) alone or HFD combined with carbon tetrachloride (CCL4) treatment (Fig. 1G, H). This evidence concerns the gene NR5A2 and metabolic dysfunction-associated steatohepatitis.