Furthermore, evaluations of single-dose influenza and rabies vaccines indicate that CXCL13 effectively enhances the protection of the single-dose influenza circRNA vaccine against heterologous influenza viruses and boosts the immunogenicity of the single-dose rabies circRNA vaccine, underscoring the potential of CXCL13 to facilitate the development of single-dose vaccines. The gene discussed is CXCL13; the disease is influenza.