The analysis revealed that the genetic disruption of the EIF4A1, EIF4E, and EIF4G1 genes encoding the eIF4A, eIF4E, and eIF4G subunits decreased fitness of most melanoma cell lines, arguing against the use of knock-out cell lines to study the impact of eIF4F inhibition in melanoma (SI Appendix, Fig. S1). The gene discussed is EIF4A1; the disease is melanoma.