CD4 and infection: On the other hand, filarial-specific CD4+ T cell responses from L. sigmodontis–infected BALB/c mice (prepared from the aforementioned two time points of the infection) were determined following stimulation ex vivo with different L. sigmodontis antigenic extracts (soluble or pellet worm fractions following standardized centrifugation or UC) prepared from adult worms either of both or individual genders.