The amputated patient group generally comprised older patients and included more men and individuals who currently smoked, consumed alcohol heavily, did not exercise regularly, had a low income, had a lower BMI, were diagnosed with hypertension and/or chronic kidney disease (CKD), were treated with insulin or more than three oral antidiabetic drugs, had higher PA scores, and had higher serum glucose levels, WBC count, systolic blood pressure, triglycerides, and high‐density lipoprotein (HDL) levels than patients without LEA. The gene discussed is INS; the disease is hypertensive disorder.