The embryos were exposed to a wide range of PPP (0.1–100,000 μg/L) by immersion and evaluated at various time periods (8–60 dpf) targeting developmental (survivability, yolk, heart, eye, tail, hatching, body length, ROS, LPO, and apoptosis), endocrinological (HPG-, HPI-, and HPT-axis and vtg), and neurobehavioral (AChE, anxiety, and thigmotaxis) endpoints. The gene discussed is ACHE; the disease is Anxiety.