To investigate whether NPC neural differentiation is affected in AD, we performed immunofluorescence to compare the number of BrdU+/DCX+(a marker for new immature neurons), BrdU+/NeuN+(a marker for new mature neurons), and BrdU+/GFAP+ (a marker for new astrocytes) cells in 3xTg and SAMP8 mice. The gene discussed is DCX; the disease is Alzheimer disease.