High TMB facilitates immunotherapy of multiple cancers.[51] Although MSI is mostly mentioned in the immunotherapy of gastrointestinal tumors,[52,53] studies of MSI evaluation of breast cancer immunotherapy have also been reported.[54] LIAS, LIPT1, and PDHB were weakly negatively correlated with TMB, while DLAT, PDHA1, GLS, and CDKN2A was weakly positively related to TMB. The gene discussed is CDKN2A; the disease is digestive system neoplasm.