Our data imply that DHEA showed a dose-dependent suppression of CRC cells regardless of the status of p53 at 72 h p.t. Furthermore, the inhibitory effect of DHEA on the anchorage-independent colony formation of three CRC cell lines (HCT116+/+, HT29, and HCT116−/−) was assessed. The gene discussed is TP53; the disease is colorectal carcinoma.