Furthermore, Zhou et al, (2019) reported that Chrysanthemulide A blockage of the fusion of the autophagosome and the lysosome transcriptionally upregulates DR5 protein in both intracellular and membrane compartments of human osteosarcoma cells, leading to apoptosis through caspase 8 activation, and silencing either LC3B or ATG5 with siRNA inhibited autophagosome formation and downregulated DR5 expression (Zhuo et al., 2019). This evidence concerns the gene CASP8 and osteosarcoma.