As shown in scRNA-seq analysis (Figure 2D), ischemic stroke injury induced higher numbers of Iba1+ microglia derived from NG2-tdT+ than physical injury did (Figure 3H-I and Figure S6C), and physical injury triggered higher numbers of Col1a1+ fibroblasts from NG2-tdT+ than ischemic stroke injury did (Figure 3J-K and Figure S6D). This evidence concerns the gene COL1A1 and ischemic stroke.