To verify whether [18F]AlF-NOTA-QHY-04 can be used for evaluation of CXCR4 expression in different types of tumors, PET imaging studies were conducted in multiple types of tumor models, including SCLC (NCI-H69), NSCLC (A549), TNBC (MDA-MB-231), glioma (U251), pancreatic carcinoma (MIA PaCa-2), and lymphoma (Daudi). Here, CXCR4 is linked to central nervous system cancer.