Furthermore, we elucidated for the first time that the high tracer uptake in certain solid patients is mainly due to the high expression of CXCR4 in tumor cells, followed by macrophages and neutrophils in the tumor tissues by mIF staining, which proved the PET signal provided by [18F]AlF-NOTA-QHY-04 indeed can accurately reflect the CXCR4 expression levels on multiple tumors. This evidence concerns the gene CXCR4 and neoplasm.