Further characterization of the EVs included the examination of the expression of the exosome biomarker Tumor Susceptibility Gene-101 (TSG101, Figure 4B), the cryo-EM imaging that verified the correct morphology and the FACS analysis of carboxyfluorescein succinimidyl ester (CFSE)-stained PDEVs to test the integrity (Figure 4C and 4D). The gene discussed is TSG101; the disease is neoplasm.