Overall, the present study focused on mast cells in esophageal cancer, and we validated the pro-carcinogenic role of this pathway by launching a comprehensive profiling of mast cell subtypes with an eye on the C1 SRSF7+ MCs and obtaining its reciprocal receptor, EGFR, using cellular communication analysis, and subsequently verifying the pro-carcinogenic role of this pathway through cellular knockdown experiments. Here, SRSF7 is linked to esophageal cancer.