Additionally, DM significantly impaired the triple (EM: IFN-γ+IL-2+TNF+), dual (CM: IFN-γ+IL-2+ and IL-2+TNF+; EM: IFN-γ+ TNF+ and IL-2+TNF+), and mono (CM: IFN-γ+, IL-2+ and TNF+; EM: IFN-γ+ and TNF+)-functional capacity of the Mtb-specific CD4+ T-cell central and effector memory responses, contributing to first evidence of DM immune impairment on polyfunctional CD4+ T-cell memory responses. Here, CD4 is linked to diabetes mellitus.