Immunohistochemical analysis showed that the tumor cells were positive for P450scc, P450c17, P450c21, and CYP11B1; faintly positive for DHEA sulfotransferase (DHEA-ST); and negative for CYP11B2 (Figs. 3A-G); confirming the cortisol-producing ability of the tumor. This evidence concerns the gene CYP11B2 and neoplasm.