HSPD1 and osteosarcoma: More importantly, the re-introduction of HSPD1 expression significantly attenuated the ATP5A1 K48-linked ubiquitination in the HSPD1-silenced osteosarcoma cells, while HSPD1 silencing obviously rescued the ATP5A1 K48-linked ubiquitination in the HSPD1-overexpressing osteosarcoma cells (Figure 9F-I).