Firstly, although we proposed an HSP-associated signature based on HSPD1, DNAJC1, DNAJC5B, and DNAJC17 and systematically evaluated its value in characterizing the immune landscape of osteosarcoma, further in vitro and ex vivo experiments are required to validate the role of these genes in regulating the TIME. This evidence concerns the gene DNAJC5B and osteosarcoma.