GSEA analysis based on HSPD1 transcript expression from all osteosarcoma patients in the training and validation cohorts showed that the positively regulated gene set by mTOR signaling (MTOR_UP.N4.V1_UP) was enriched significantly in HSPD1-overexpressing cohorts (Figure 7B). The gene discussed is HSPD1; the disease is osteosarcoma.