Incorporating DFS scores of tumors and NATs, MVI, tumor diameter, AFP, differentiation, and Serum HBV-DNA, we further verified that the DFS score of the NATs is an independent prognostic risk factor relative to the tumor and clinical features through multiple Cox regression analysis (N.DFS.socre-Xing's cohort: multivariate hazard ratio = 2.389; P-value < 0.001; Supplementary Table S4). The gene discussed is AFP; the disease is neoplasm.