In order to gain a better understanding of the mechanisms by which HNF4A-AS1 influences the progression of HCC cells, the researchers conducted RNA sequencing to investigate the transcriptional profiles of SK-Hep1 cells that were overexpressing HNF4A-AS1 (Figure 7A, B, Supplemental Table 7). The gene discussed is HNF4A; the disease is hepatocellular carcinoma.