Firstly, we utilized lentiviral transfection to ectopically express USP44 in a panel of thyroid cancer cell lines, including SW1736 (ATC, p53-/-), BCPAP (PTC, p53mut), 8505C (ATC, p53mut), K1 (PTC, p53wt) and IHH4 (ATC, p53wt). The gene discussed is TP53; the disease is thyroid cancer.